Abstract
Purpose: A male accessory gland 31.7 kDa protein (MAG-317) was purified and characterized from the mosquito Aedes aegypti (L.) by SDS-PAGE and Coomassie brilliant blue staining. Methods: The specificity of the polyclonal antibodies raised in rabbit to the purified MAG-317 protein was verified by immunoblot analysis of crude male accessory gland (MAG) extracts and the protein was separated by SDS-PAGE. Results: The N-terminal amino acid sequence of the purified MAG-317 has 79% identity to the derived amino terminal sequence of the CG6041 gene product of Drosophila melanogaster and 47% identity to the derived amino terminal sequence of a 3al cDNA of midgut of Aedes aegypti which encodes for a putative trypsinogen. MAG-317 was a soluble protein that was detected only in the supernatant of tissue homogenate. For in situ localization of the protein, confocal laser-scanning microscope was used to observe cross-sections of the MAG-317 was found to distribute in the whole accessory gland. Subsequently labeled the male mosquitoes with the fluorescence isothiocynate conjugated MAG-317 in vivo and allowed these males to copulate with virgin females showed that MAG-317 could be detected in female spermathecae. Conclusion: Our study concludes that MAG-317 can be transferred from male to the female mosquito during copulation.
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