A major 170 kDa protein associated with bovine adrenal medulla microtubules: a member of the centrosomin family?

Abstract

Microtubules isolated from bovine adrenal medulla cells contain a major 170 kDa protein (p170). p170 is heat-labile and is associated with microtubules in an ATP-insensitive manner. This protein was purified to near homogeneity using FPLC. A preparation containing purified p170 caused bundling of microtubules. By microsequencing of p170, two polypeptides were identified which appeared to be identical to a recently sequenced p167 centrosomin-related protein. Polyclonal affinity-purified anti-p170 antibody was found to immunostain microtubules and to recognize the 170 kDa polypeptide in culture cells. We suggest that p170 is a new member of a centrosomin family and is a new structural protein associated with microtubules in some cell types.