A low-toxicity baiting program precipitates collapse of Argentine ant and ant-associated hemipteran pest populations in commercial citrus

Abstract

The Argentine ant, Linepithema humile Mayr (Hymenoptera: Formicidae), disrupts biological control agents which induces population outbreaks of honeydew-producing hemipteran pests infesting agroecosystems. A multi-season liquid baiting program was evaluated for control of L. humile and ant-associated hemipteran pests in six commercial citrus orchards in southern California. Ant activity in treated trees fell by 96% within three months of treatment and was, on average, 95% lower in treated plots than untreated plots across the 18-month study period. Ant control resulted in the near elimination of hemipteran pest populations on trees in treated plots. Pre- and post-treatment estimates indicated that hemipteran pest infestation of twigs, flush, and fruit decreased by 97, 84, and 99% one year after treatment, respectively. Furthermore, cumulative infestation frequency was 10 times lower and cumulative colony size was 5 times lower on treated trees than control trees across the final year of study. Examining individual hemipteran pest species, the mean infestation rate by Diaphorina citri Kuwayama (Hemiptera: Liviidae) was 75% lower, Coccus hesperidum L. (Hemiptera: Coccidae) 95% lower, Planococcus citri Risso (Hemiptera: Pseudococcidae) 98% – 100% lower, and Aonidiella aurantii Maskell (Hemiptera: Diaspidae) 70 – 80% lower in treated trees relative to untreated trees. A surge in generalist predator activity and parasitism following ant bait deployment appear to be the driving forces behind the marked decrease in pest infestation observed in treated trees. Results underscore the importance of L. humile control for integrated pest management programs targeting ant-associated hemipteran pests in commercial citrus.