Abstract
AbstractViroid‐free potato and chrysanthemum plants were obtained from meristem‐tips cut from potato spindle tuber viroid‐infected potato plants and from chrysanthemum plants infected with chrysanthemum stunt, chrysanthemum chlorotic mottle or cucumber palefruit viroids after 6 months therapy in a growth chamber at 5 °C and 16 hours daily light of 5.000 lx intensity. Chrysanthemum plants survived quite well the conditions of therapy while potato plants grown from stem cuttings survived these conditions much worse and potato plants grown from tubers did not survive these conditions. PSTV‐free plants were obtained from meristem‐tips cut from sprouts grown from potato tubers infected with severe (s‐PSTV) or mild (m‐PSTV) strains of potato spindle tuber viroid after 6 months therapy at 6–7 °C in the dark. The tubers survived these conditions quite well.The 3 months therapy period was found too short for any plant material. The efficiency of 6 months therapy in viroid elimination varied for different viroids and different plant material from 18.5 to 80.0 %.
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