A low-molecular-weight ascophyllan prepared from Ascophyllum nodosum: Optimization, analysis and biological activities

Abstract

In this study, a low-molecular-weight saccharide fragment (LMWAs-L) was prepared from alginate lyase (EC 4.2.2.3) hydrolyzed ascophyllan by ultra-filtration separation method. LMWAs-L was a homogeneous saccharide fraction with an average molecular weight of 6.96 kDa. Enzymolysis process optimization experiments revealed that the optimum process parameters for preparing LMWAs-L were the enzyme concentration 0.02 U/mL, initial pH 6.8, and enzymolysis temperature 43 °C. After optimization, the yield of LMWAs-L was increased to 9.74% higher than that without optimization. Interestingly, LMWAs-L exhibited stronger enhancing activities on the proliferation and migration of human skin fibroblasts cells in vitro and better antibacterial activities as compared to native ascophyllan at the same mass concentration. Our study establishes a simple way to prepare low-molecular-weight saccharide with beneficial bioactivities from ascophyllan efficiently. This is the first report to reveal that ascophyllan and its low-molecular-weight saccharide have the potentials to be developed as natural biological dressing and antibacterial agents.