A look into osmotic, ionic, and redox adjustments in wild tomato species under combined salt and water stress

Abstract

Conventional tomato production is widely threatened by environmental changes that impose increasingly frequent and severe conditions of soil salinization and water shortage. The assessment of the wild germplasm has become an appealing strategy for the stress-resilience improvement of crops. Tomato interspecific diversity encompasses wild species that are native to the dry shores and high-elevated deserts of the Andean countries, often thriving under circumstances of drought and salinity. The present work aimed to compare the effects of moderate salinity stress under different watering regimes on the ion distribution, redox homeostasis, osmoregulation, and antioxidant defenses between a domestic cultivar of tomato (Chico III) and the wild tomato species Solanum galapagense (LA1403), Solanum habrochaites (LA1223), and Solanum neorickii (LA2194). Results showed that although wild tomato plants grew slower than the cultivar, their growth was less affected by exposure to salt or to lower water availability. S. galapagense revealed a Na+ includer behavior under salt stress, increasing Na+ levels by 6-fold over control, reaching levels 4 times higher than in the cultivar. Nonetheless, H2O2-detoxifying enzymes were activated, and shoot elongation was sustained in this species, suggesting an efficient Na+ compartmentalization. On the other hand, the domestic cultivar had the highest accumulation of Na+ in roots and showed the lowest ability to sustain growth under combined stress. Leaves of S. habrochaites showed a huge proline buildup under salt stress, whereas S. neorickii and S. galapagense seemed to prevent proline accumulation. S. habrochaites also had high levels of antioxidant metabolites and superoxide dismutase activity under control conditions but downregulated further antioxidant defenses in response to stress exposure. No oxidative damages were noticed despite the almost 2-fold increase in ROS content in the leaves of S. neorickii under salt stress, which showed a negative correlation with growth traits, but an improvement in the antioxidant potential. A principal component analysis (PCA) revealed five PCs with eigenvalues >1, explaining 84 % of the total variability, and suggesting a separation of the evaluated samples mainly in accordance with the type of redox disturbances and antioxidant defenses employed, levels of photosynthetic pigments, balance between Na+ and K+ uptake and proline accumulation. These findings show that wild tomato plants respond differently than cultivated ones under moderate salinity and reduced water availability, suggesting interesting osmoregulatory and antioxidant mechanisms in S. galapagense and S. habrochaites.