A live attenuated strain of HY9901ΔdctP provides protection against Vibrio alginolyticus to the pearl gentian grouper (♀Epinephelus fuscoguttatus × ♂Epinephelus lanceolatus)

Abstract

In recent decades, vibriosis caused byVibrio alginolyticushas become a severe threat to the global mariculture industry. There is an urgent need for an effective vaccine to alleviate this unoptimistic situation. In this study, we evaluated the safety, immunoprotection, and specific and non-specific immune response effect of ΔdctPstrain as a live-attenuated vaccine to pearl gentian grouper (♀Epinephelus fuscoguttatus× ♂Epinephelus lanceolatu). The results demonstrate that the safe dose of ΔdctPwas ≤1.0 ×106CFU in pearl gentian grouper. The relative percent survival of the pearl gentian grouper challenged with the ΔdctPmutant strain by intraperitoneal injection reached 74.4%, which was significantly higher than that of the control group. Meanwhile, the expression level of immune-relative genes, includingIgM,IL-1β,IL-8,IL-10,MHC-Iα,MHC2,TNF-α,TLR3, andCD4, were upregulated in liver, spleen, and head kidney within 28 d post-vaccination. Moreover, specific antibody IgM, total serum protein as well as activities of catalase, superoxide dismutase, and lysozyme in serum were significantly up-regulated in vaccinated groupers compared with those in control. Collectively, ΔdctPcould be used as a live-attenuated vaccine candidate againstV. alginolyticusinfection in pearl gentian grouper.