Abstract
It was demonstrated that amino acids, in spite of their strongly hydrophilic character, distribute between water and a mixed solvent composed of n-hexanol and di(2-ethyl-hexyl) orthophosphoric acid. The mixed organic phase, belonging to the class of liquid ion exchangers, is practically immiscible with water; the solvent system can thus be employed in column liquid-liquid partition chromatography. The partition of amino acids in the system studied can be controlled by pH of the aqueous phase and/or concentration of HDEHP in the organic phase.
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