Abstract
Abstract Lipid-soluble DC Silicone Fluid 703 (a methyl-phenyl polysiloxane) was tested as an inert indicator in fat absorption studies. The recovery of this fluid in rat stools was 96.0 ± 6.7 per cent (mean ± S.D.). This agreed with the recovery in the in vitro experiment, 93.8 ± 4.0 per cent, where known amounts of DC Silicone Fluid 703 were added to rat feces and analyzed. In the amounts tested (75, 150, and 300 mg.), DC Silicone Fluid 703 had no influence on the absorption of glyceryl-tristearate-1-C 14 in olive oil. The absorption of this mixture determined by the aid of DC Silicone Fluid 703 as an inert indicator and by the complete collection of the stool was 88.8 ± 3.0 per cent and 89.2 ± 3.6 per cent, respectively. From these results DC Silicone Fluid 703 was considered to be a suitable inert indicator for use in fat absorption studies.
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