Abstract
BackgroundBladder tissue engineering is an excellent alternative to conventional gastrointestinal bladder enlargement in the treatment of various acquired and congenital bladder abnormalities. We constructed a nanosphere-small MyoD activating RNA-bladder acellular matrix graft scaffold NP(saMyoD)/BAMG inoculated with adipose-derived stem cells (ADSC) to explore its effect on smooth muscle regeneration and bladder repair function in a rat augmentation model.MethodsWe performed many biotechniques, such as reverse transcriptase-polymerase chain reaction (RT-PCR), Western blot, MTT assay, HE staining, masson staining, and immunohistochemistry in our study. Lipid nanospheres were transfected into rat ADSCs after encapsulate saRNA-MyoD as an introduction vector. Lipid nanospheres encapsulated with saRNA-MyoD were transfected into rat ADSCs. The functional transfected rat ADSCs were called ADSC-NP(saMyoD). Then, Rat models were divided into four groups: sham group, ADSC-BAMG group, ADSC-NP(saMyoD)/BAMG group, and ADSC-NP(saMyoD)/SF(VEGF)/BAMG group. Finally, we compared the bladder function of different models by detecting the bladder histology, bladder capacity, smooth muscle function in each group.ResultsRT-PCR and Western blot results showed that ADSCs transfected with NP(saMyoD) could induce high expression of α-SMA, SM22α, and Desmin. At the same time, MTT analysis showed that NP(saMyoD) did not affect the activity of ADSC cells, suggesting little toxicity. HE staining and immunohistochemistry indicated that the rat bladder repair effect (smooth muscle function, bladder capacities) was better in the ADSC-NP(saMyoD)/BAMG group, ADSC-NP(saMyoD)/SF(VEGF)/BAMG group than in the control group.ConclusionsTaken together, our results demonstrate that the NP(saMyoD)/SF(VEGF)/BAMG scaffold seeded with ADSCs could promote bladder morphological regeneration and improved bladder urinary function. This strategy of ADSC-NP(saMyoD)/SF(VEGF)/BAMG may has a potential to repair bladder defects in the future.
Highlights
Reconstruction of the bladder is imminent in various congenital and acquired urinary tract diseases, such as neuro-bladder, bladder cancer, and congenital bladder abnormalities (Lam Van Ba et al, 2015)
The results showed that 85.12% of the detected isolated cells were able to express CD90, 78.87% express CD29 and 81.54% express CD105 (Schäffler and Büchler, 2007; Yamamoto et al, 2007), but few isolated cells express CD34 (4.52%)
These results strongly suggested that we had isolated and culture adiposederived stem cells (ADSC) successfully
Summary
Reconstruction of the bladder is imminent in various congenital and acquired urinary tract diseases, such as neuro-bladder, bladder cancer, and congenital bladder abnormalities (Lam Van Ba et al, 2015). In the context of the imminent need for new alternatives to entero-cystoplasty, the augmentation of tissue-engineered (TE) bladder brings a new approach to bladder reconstruction (Adamowicz et al, 2019). Bladder acellular matrix graft (BAMG) has been proposed as a preferable scaffold with the same extracellular matrix composition, mechanical properties and complexity, comparing with native tissue (Coutu et al, 2014). We constructed a nanosphere-small MyoD activating RNA-bladder acellular matrix graft scaffold NP(saMyoD)/BAMG inoculated with adiposederived stem cells (ADSC) to explore its effect on smooth muscle regeneration and bladder repair function in a rat augmentation model
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
