A line-scan hyperspectral system for high-throughput Raman chemical imaging.

Abstract

A line-scan hyperspectral system was developed to enable Raman chemical imaging for large sample areas. A custom-designed 785 nm line laser based on a scanning mirror serves as an excitation source. A 45° dichroic beam splitter reflects the laser light to form a 24 cm × 1 mm excitation line normally incident on the sample surface. Raman signals along the laser line are collected by a detection module consisting of a dispersive imaging spectrograph and a CCD camera. A hypercube is accumulated line by line as a motorized table moves the samples transversely through the laser line. The system covers a Raman shift range of -648.7 to 2889.0 cm(-1) and a 23 cm wide area. An example application for authenticating milk powder is presented to demonstrate the system performance. In 4 min the system acquired a 512 × 110 × 1024 hypercube (56 320 spectra) from four, 47 mm diameter Petri dishes containing four powder samples. Chemical images were created for detecting two adulterants (melamine and dicyandiamide) that had been mixed into the milk powder.