A Light-Driven InVitro Enzymatic Biosystem for the Synthesis of α-Farnesene from Methanol.

Abstract

Terpenoids of substantial industrial interest are mainly obtained through direct extraction from plant sources. Recently, microbial cell factories or invitro enzymatic biosystems have emerged as promising alternatives for terpenoid production. Here, we report a route for the synthesis of α-farnesene based on an invitro enzyme cascade reaction using methanol as an inexpensive and renewable C1 substrate. Thirteen biocatalytic reactions divided into 2 modules were optimized and coupled to achieve methanol-to-α-farnesene conversion via integration with natural thylakoid membranes as a green energy engine. This invitro enzymatic biosystem driven by light enabled the production of 1.43 and 2.40 mg liter-1 α-farnesene using methanol and the intermediate glycolaldehyde as substrates, respectively. This work could provide a promising strategy for developing light-powered invitro biosynthetic platforms to produce more natural compounds synthesized from C1 substrates.