A lentiviral vector encoding fusion of light invariant chain and mycobacterial antigens induces protective CD4+ Tcell immunity.

Abstract

Lentiviral vectors (LVs) are highly efficient at inducing CD8+ Tcell responses. However, LV-encoded antigens are processed inside the cytosol of antigen-presenting cells, which does not directly communicate with the endosomal major histocompatibility complex class II (MHC-II) presentation pathway. LVs are thus poor at inducing CD4+ Tcell response. To overcome this limitation, we devised a strategy whereby LV-encoded antigens are extended at their N-terminal end with the MHC-II-associated light invariant chain (li), which contains an endosome-targeting signal sequence. When evaluated with an LV-encoded polyantigen composed of CD4+ Tcell targets from Mycobacterium tuberculosis, intranasal vaccination in mice triggers pulmonary polyfunctional CD4+ and CD8+ Tcell responses. Adjuvantation of these LVs extends the mucosal immunity to Th17 and Tc17 responses. A systemic prime and an intranasal boost with one of these LV induces protection against M.tuberculosis. This strategy improves the protective power of LVs against infections and cancers, where CD4+ Tcell immunity plays an important role.