A Leaderless Two-Peptide Bacteriocin, Enterocin DD14, Is Involved in Its Own Self-Immunity: Evidence and Insights.

Rabia Ladjouzi,Djamel Drider,Anca Lucau-Danila,Abdellah Benachour

doi:10.3389/fbioe.2020.00644

Abstract

Enterocin DD14 (EntDD14) is a two-peptide leaderless bacteriocin produced by Enterococcus faecalis 14, a strain previously isolated from meconium. EntDD14 has a strong antibacterial activity against Gram-positive bacteria. Leaderless bacteriocins, unlike bacteriocins with leader peptides, are immediately active after their translation, and a producing strain has then to develop specific mechanisms to protect both intra and extracellular compartments. The in silico analysis of Ent. faecalis 14 genome allowed to locate downstream of structural ddAB genes, 8 other adjacent genes, designed ddCDEFGHIJ, which collectively may form three operons. To gain insights on immunity mechanisms of Ent. faecalis 14, mutant strains knocked out in ddAB genes encoding bacteriocin precursor peptides (Δbac) and/or ABC transporter (ΔddI) of EntDD14 were constructed and characterized. Importantly, Δbac mutant strains, from which structural ddAB genes were deleted, resulted unable to produce EntDD14 and sensitive to exogenous EntDD14 showing their involvement in the Ent. faecalis 14 immunity system. Moreover, the sensitivity of Δbac mutants appeared not to be associated with the down-regulation of ddCDEFGHIJ gene expression since they were similarly expressed in both Δbac and wild-type strains during the log phase while they were found significantly down-regulated in the Δbac mutant strain after 24 h of growth. Data gathered from this study suggest also the implication of the ABC transporter (ddHIJ) in the active export of EntDD14 but ruled-out its involvement in the primary self-immunity system. Interestingly, non-bacteriocin producing Ent. faecalis JH2-2 cells transformed with ddAB, or ddAB plus genes encoding the ABC transporter (ddAB-HIJ) did not produce EntDD14 and remained sensitive to its action. Of note, trans-complementation of the Δbac mutant strain with these constructions allowed to recover the WT phenotype. To the best of our knowledge, this is the first study delineating the role of the intracellular two-peptide leaderless bacteriocins in their self-immunity.

Highlights

Enterococci are members of the lactic acid bacteria (LAB) group that are known to produce potent bacteriocins, named enterocins
In silico analysis of the genetic neighborhood of the Enterocin DD14 (EntDD14) structural genes revealed a cluster of 10 open reading frames (ORFs) ddABCDEFGHIJ (Figure 1)
All the bacteriocinogenic strains above-cited share at least a set of 3 genes, which are highly homologous to the second ABC transport system (As-48FGH) described for the cyclic bacteriocin AS-48 (Diaz et al, 2003) (Figure 1)

Summary

Introduction

Enterococci are members of the lactic acid bacteria (LAB) group that are known to produce potent bacteriocins, named enterocins. Many studies have reported the promising potential of bacteriocins for use in in different areas like food industry (Perez et al, 2014; Juturu and Wu, 2018), and both clinical and veterinary applications (Papo and Shai, 2005; Diez-Gonzalez, 2007; van Heel et al, 2011; Hu et al, 2018). According to their physicochemical properties, bacteriocins can cause perturbations of cell membranes (pore formation, followed by efflux of metabolites and ions), depolarization of membranes, inhibition of cell wall synthesis or septum formation, disruption of DNA replication and transcription, or interference at the ribosomal level preventing protein synthesis (Cotter et al, 2013; Verma et al, 2014; Johnson et al, 2018). Class I contains small post-translationally modified peptides, designed RiPPs (ribosomally synthesized, post-translationally modified peptides), and are

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