Abstract
Allergies are highly complex disorders with clinical manifestations ranging from mild oral, gastrointestinal, recurrent wheezing, and cutaneous symptoms to life-threatening systemic conditions. The levels of arachidonic acid, eicosanoids, histamine, organic acids and valine are considered to have a variety of physiological functions in connection with allergies. In this research, we have developed a RP-LC/MS method to separate and quantitate six different potential endogenous biomarkers, including leukotrieneB4 (LTB4), prostaglandinD2 (PGD2), arachidonic acid (AA), histamine (HI), lactic acid (LA) and valine (VAL), from serum of rats with ovalbumin (OVA)-induced allergy and normal rats, and the discrepancies between the model group and the control group were compared. The separation was performed on a Prevail C18 column (250 mm × 4.6 mm, 5 μm) with a gradient elution of acetonitrile with 0.1% formic acid (v/v) and 10 mM ammonium formate (adjusted to pH 4.0 with formic acid) at a flow rate of 0.5 mL min−1 The method was validated and shown to be sensitive, accurate (recovery values 76.16–92.57%) and precise (RSD < 10% for all compounds) with a linear range over several orders of magnitude. The method was successfully applied to rat serum and shown to be indicative of the endogenous levels of biomarkers within the rat body. The analysis of the biomarkers can provide insight into the allergic mechanisms associated with related diseases.
Highlights
Allergies can cause significant morbidity in children and several studies have revealed that their prevalence may be increasing, especially asthma [1,2,3,4]
We established an OVA-induced allergy model with BN rats, and developed a RP-LC/MS method to separate and quantitate six different potential endogenous biomarkers (LTB4, PGD2, arachidonic acid (AA), HI, lactic acid (LA) and VAL), which have physiological functions in allergic inflammation, from serum of allergic rats induced by ovalbumin (OVA) and normal rats, and compared the discrepancies between the model group and the control group
AA and AA-derived eicosanoids are potent pro- and anti-inflammatory mediators, which play an important role in the pathogenesis of allergic inflammation
Summary
School of Chinese Materia Medica, Beijing University of Chinese Medicine, No.6, Zhonghuan Science and Technology Development Center for Traditional Chinese Medicine in Beijjing Received: 3 February 2010; in revised form: 13 April 2010 / Accepted: 19 April 2010 /
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