A large scale preparative gel electrophoresis separation of alpha 1 and alpha 2 subunits of the voltage-gated Ca2+ channel from rabbit skeletal muscle.

Abstract

A large-scale preparative gel electrophoresis method effectively separates individual voltage-gated calcium channel (VGCC) subunits with high resolution, starting with up to 4 mg of rabbit skeletal muscle L-type VGCC complex. Using this method, we separated alpha 1 and alpha 2 subunits of rabbit skeletal muscle VGCC with a high efficiency and with protein recoveries of 83%. The separated alpha 1 and alpha 2 subunits eluted from the gel in a 1:1 molar ratio. The method should be applicable for separating the other VGCC subunits or subunits of other protein complexes.