A β-lactamase belonging to group 2e from oral clinical isolates of Prevotella intermedia

Abstract

A β-lactamase in oral clinical isolates of Prevotella intermedia that hydrolyzed cefuroxime and cephalothin with rates of 600 and 53.3 respectively, relative to that for cephaloridine (100), was characterized as a 2e-cephalosporinase. Inhibition was observed by clavulanic acid (IC 50 0.72 μM), tazobactam (IC 50 0.21 μM) and sulbactam (IC 50 0.07 μM) and was not inhibited by cloxacillin, EDTA, NaCl or p-CMB. The p I and pH optima were 4.7 and 5.4, respectively.