A LABORATORY EXERCISE TO DEMONSTRATE DIRECT AND INDIRECT SHOOT ORGANOGENESIS FROM LEAVES OF TORENIA FOURNIERI

Abstract

Plant cell cultures have the ability to regenerate roots, shoots, leaves, and flowers de novo. The production of adventitious shoots in vitro is easy to control on Torenia fournieri (Wishbone Flower). Direct shoot organogenesis is possible to obtain from leaf explants without an intervening callus phase. Leaf explants should be placed on a Murashige and Skoog (MS) basal medium with 6-benzylaminopurine (BAP) levels from 2.5-5 mg/1 and indole-3-butyric acid (IBA) at 0.1 mg/liter for direct shoot organogenesis. If leaf explants are placed on a MS medium with 1 mg 2,4-dichlorophenoxyacetic acid (2,4-D) per liter, callus formation will occur. If the callus is subcultured onto a MS medium with 0.5-1.0 mg BAP per liter and 0.1 mg IBA per liter, indirect shoot organogenesis will occur.