Abstract
Isotope labeling with a 13CO 2-enriched atmosphere was conducted on four raised beds of grain sorghum. A temporary rain shelter with clear polyethylene roofing material was constructed to allow controlled watering of the beds. Labeling chambers were constructed with aluminum frames, polyethylene sides and propofilm C tops. The chamber length (4 m) was one-quarter of the total bed length and enclosed a total volume of ∼ 3000 1. A fan recirculated air in the chamber through polyvinyl chloride pipes. An outlet allowed sampling of chamber atmosphere for CO 2 concentration, while another opening downstream provided an inlet for 13CO 2 injection. An elebated extension was added when the grain sorghum grew to exceed the 50-cm height of the chamber, doubling the enclosed volume. Enrichment of the chamber atmosphere with 13CO 2 was monitored with infrared gas analyzers. Gas flowmeters were used to measure isotope additions.
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