Abstract
Herein we described a deoxyribonucleic acid (DNA) calculator for sensitive detection of the determination of adenosine triphosphate (ATP) using gold nanoparticles (GNP) and PicoGreen fluorescence dye as signal transducer, and ATP and single-stranded DNA (DNA-M′) as activators. The calculator-related performances including linearity, reaction time, logic gate, and selectivity were investigated, respectively. The results revealed that this oligonucleotide sensor was highly sensitive and selective. The detection range was 50–500 nmol/L (R2 = 0.99391) and the detection limit was 46.5 nmol/L. The AND DNA calculator was successfully used for the ATP detection in human urine. Compared with other methods, this DNA calculator has the characteristics of being label-free, non-enzymic, simple, and highly sensitive.
Highlights
Many diseases, such as Parkinson’s disease, ischemia, hypoxia, hypoglycemia, and some malignant tumors, are closely related to the concentration of adenosine triphosphate (ATP) [1,2,3]
We investigated the use of gold nanoparticles (GNP) and the PicoGreen probe to construct a biosensor for ATP based on label-free fluorescence
Adenosine diphosphate (ADP), adenosine monophosphate (AMP), Cytidine triphosphate (CTP), ATP, guanosine triphosphate (GTP), uridine triphosphate (UTP), and PicoGreen dye were supplied by Shanghai Yi Sheng Technology Co., Ltd. (Shanghai, China)
Summary
Many diseases, such as Parkinson’s disease, ischemia, hypoxia, hypoglycemia, and some malignant tumors, are closely related to the concentration of ATP [1,2,3]. Considerable efforts are being made to develop fluorescent sensors for the detection of ATP. Signal amplification techniques, such as rolling circle replication [5] and strand displacement amplification [6,7,8,9], provide an effective strategy for improving the sensitivity of various biosensors. These methods usually suffer from a high background signal and often rely on the use of biological enzymes and fluorescent labels. The approach of background signal suppression is often simpler and requires a shorter reaction time [10,11]
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