Abstract
In this article, a highly sensitive label-free immunosensor based on a graphene oxide (GO)/Fe3O4/Prussian blue (PB) nanocomposite modified electrode was developed for the determination of human hepatitis B surface antigen (HBsAg). In this electrochemical immunoassay system, PB was used as a redox probe, while GO/Fe3O4/PB nanocomposites and AuNPs were prepared and coated on screen-printed electrodes to enhance the detection sensitivity and to immobilize the hepatitis B surface antibody (HBsAb). The immunosensor was fabricated based on the principle that the decrease in peak currents of PB is proportional to the concentration of HBsAg captured on the modified immunosensor. The experimental results revealed that the immunosensor exhibited a sensitive response to HBsAg in the range of 0.5 pg·mL−1 to 200 ng·mL−1, and with a low detection limit of 0.166 pg·mL−1 (S/N = 3). Furthermore, the proposed immunosensor was used to detect several clinical serum samples with acceptable results, and it also showed good reproducibility, selectivity and stability, which may have a promising potential application in clinical immunoassays.
Highlights
The hepatitis B virus (HBV) has become a leading cause of death worldwide [1]
Once you have been infected with chronic hepatitis B and this is not been arrested in a timely manner and effectively, it will further evolve into acute hepatitis, chronic hepatitis and cirrhosis, and will even lead to serious complications such as liver cancer, eventually leading to death [4]
Electrochemical impedance voltammetry (CV), differential pulsepulse voltammetry (DPV) and electrochemical impedance spectroscopy spectroscopy (EIS).inThe in the peak was proportional to the concentrations of hepatitis B surface antigen (HBsAg) (EIS)
Summary
The hepatitis B virus (HBV) has become a leading cause of death worldwide [1]. Under the influence of the nano-effect, Fe3 O4 nanomaterials with different morphologies have different properties, which makes it possible to prepare some new functional materials with special performance requirements [30]. They are widely used in electromagnetics, the chemical industry, catalysis, sensors, acoustics, medicine, environmental protection and other related fields. A novel label-free electrochemical immunosensor based on redox-active conductive. The proposed method can be employed to detect HBsAg in real human serum samples with satisfactory results, which provides promising potential applications in clinical immunoassays
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