Abstract

Abstract Cadmium is considered one of the most toxic pollutants that can be found in water as well as in soil, which might accumulate in living organisms causing severe effects such as skeletal, cardiovascular, and respiratory diseases. Hence, it is necessary to develop methods allowing for sensitive and fast detection as well as portability. One possibility is the application of biosensors containing aptamer strands as receptor layer selective towards cadmium ions. Here, we present studies on the utilization of DNA aptamer strand for fabrication of sensing layer toward cadmium ions on gold disk macroelectrodes. It is shown that proposed aptasensor enables Cd2+ ions detection in the range from 10 to 50 nM with LOD of 9.5 nM and exhibits high selectivity towards cadmium cations with a response at least two times higher than that for interfering ions. Moreover, studies on the stability revealed that sensing layer preserved its binding properties after storage and allowed for cadmium ions detection in the range from 10 to 50 nM and the aptamer sensing layer could be regenerated and applied for another set of analysis of cadmium ions.

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