Abstract
The paper presents the results of laboratory trials of method using complex initial testing of antibodies to six agents of hemotransmissible infections: HIV, hepatitis B and C viruses, cytomegalovirus, Treponema pallidum and Toxoplasma gondii. The method is based on multiplexed dot immunoassay using plane protein arrays (immunochips) with the use of colloidal gold conjugates and silver development. We describe composition of the kit enabling us to conduct dot assay at room temperature within 70 min. It was shown that the results of multiplexed analysis correlate well with data obtained using commercially available monospecific kits for ELISA. Furthermore, multiplexed dot assay is faster and more efficient in execution as compared to ELISA and can be carried out in field conditions.
Highlights
Among broad range of agents of infectious diseases transmitted with blood hepatitis B (HBV) and hepatitis C (HCV) viruses, Human Immunodeficiency Virus (HIV), as well as syphilis agent (T. pallidum) are the most prominent (Schreiber et al, 1996)
Cytomegalovirus (CMV) (Volkova et al, 2015) and toxoplasmosis agent (T. gondii) are of importance with their reproduction connected with cell blood elements
We reported on the development of methodology for complex detection of antibodies to TORCH diseases (Poltavchenko and Yakovchenko, 2007) and evaluation of postvaccinal immunity to childhood diseases (Ersh et al, 2015) using multiplexed dot immunoassay based on plane protein arrays
Summary
Among broad range of agents of infectious diseases transmitted with blood hepatitis B (HBV) and hepatitis C (HCV) viruses, Human Immunodeficiency Virus (HIV), as well as syphilis agent (T. pallidum) are the most prominent (Schreiber et al, 1996). Hemotransmissible infections are widely spread among population and are able to lead to dire consequences. Course of these diseases has often the form of mixed infections in different combinations and lacks specific symptoms (Zhiburt, 2002). Rapid and efficient detection of hemotransmissible infections is one of the important stages to control them including mandatory medical health screening of donors and groups of population with increased risk of contamination. The monitoring is carried out by means of screening or diagnostic testing. Differences among those processes are caused by tasks for analyses conduction, as well as subsequent stages with their techniques being similar to each other (WHO, 2009)
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