Abstract
In order to determine the kinetic parameters of glucose oxidation catalysed by the enzyme glucose oxidase (GO) the initial velocity of hydrogen peroxide formation was measured using a rotating disc electrode (RDE). The major advantage of this method is the possibility of continuous measurement of the increase in hydrogen peroxide concentration. This means that the real initial reaction rate V 0 can be determined, which is required for constructing a double-reciprocal plot. Several combinations of substrate concentrations (glucose and oxygen) were used. The method, in which a platinum black RDE was used, appeared to be very useful. Product inhibition experiments showed that the ping-pong mechanism is valid for GO. The three kinetic parameters of this mechanism were determined by initial velocity experiments.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
