Abstract
Previous research on Hypsipyla robusta revealed that females release and produce a three-component sex pheromone that consists of a 5:3:2 blend of (Z,E)-9,12-tetradecadienyl acetate (Z9E12-14:Ac), (Z)-9-tetradecenyl acetate (Z9-14:Ac) and (Z)-11-hexadecenyl acetate (Z11-16:Ac). However, the lure based on this formula was not effective or had no attraction to male H. robusta although used for monitoring H. robusta populations in the some field tests. We examined the sex pheromone active components of H. robusta in Guangdong, China. The pheromone gland extracts of female moths were analyzed by gas chromatography coupled with mass spectroscopy (GC–MS) and gas chromatography with electroantennographic detection (GC–EAD). GC–MS and GC–EAD analyses of courtship females (aerations) detected a new compound, (Z)-9-tetradecen-1-ol (Z9-14:OH). When Z9-14:OH was combined with Z9E12-14:Ac, Z9-14:Ac and Z11-16:Ac, the lures were attractive to male moths. The results suggested that Z9-14:OH was an important component in H. robusta female sex pheromone communication system. Adding Z9-14:OH to sex pheromone lure formulations would improve the efficacy of pheromone trap activity in China.
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