Abstract
Embryonic chick ciliary ganglion (CG) neurons obtained from E7–E8 ganglia maintained in serum-free medium were stimulated with 50 μM muscarine. A fast hyperpolarization of the membrane potential was observed in 25% of the cells tested, that in some cases was associated with a slower depolarization. Accordingly, in voltage clamp experiments, either an outward current or a biphasic current response could be observed. Single-channel experiments provide evidence that these signals can be associated to the activation of a K + channel whose conductance is 20 pS.
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