Abstract

The hupL gene, encoding the uptake hydrogenase large subunit, in Nostoc sp. strain ATCC 29133, a strain lacking a bidirectional hydrogenase, was inactivated by insertional mutagenesis. Recombinant strains were isolated and analysed, and one hupL − strain, NHM5, was selected for further study. Cultures of NHM5 were grown under nitrogen-fixing conditions and H 2 evolution under air was observed using an H 2 electrode.

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