A HYDROGEN PEROXIDE SENSOR BASED ON PRUSSIAN BLUE AND DNA-MODIFIED ELECTRODE

Abstract

The polyvinylpyrrolidone (PVP) protected Prussian blue (PB) nanoparticles were prepared for use as a sensor for the catalytic reduction of hydrogen peroxide. The nanoparticles were used to modify a gold electrode, which was applied to detect hydrogen peroxide. Impedance spectra and differential pulse voltammetry were applied to detect the performance of the novel electrode and its response to H2O2 and Ramos cell smash fluid. Under the optimized experimental conditions, hydrogen peroxide was detected in a linear range of 6.25 × 10−7 to 1.0 × 10−5 mol · L−1, and the detection limit was 7.12 × 10−8 mol · L−1 according to 3σ rule. Under the same conditions, hydrogen peroxide was determined in Ramos cell smash fluid with a linear range from 400–80000 mol mL−1, with a detection limit of 114 mL−1 according to 3σ rule. The modified electrode with Prussian blue nanoparticles displayed high sensitivity, good reproducibility, and long-term stability to hydrogen peroxide.