Abstract
BackgroundBilophila wadsworthia is a major member of sulfidogenic bacteria in human gut, it was originally recovered from different clinical specimens of intra-abdominal infections and recently was reported potentially linked to different chronic metabolic disorders. However, there is still insufficient understanding on its detailed function and mechanism to date.MethodsA B. wadsworthia strain was isolated from fresh feces of a latent autoimmune diabetes in adults patient and we investigated its pathogenicity by oral administration to specific-pathogen-free mice. Tissue samples and serum were collected after sacrifice. Stool samples were collected at different time points to profile the gut microbiota.ResultsBilophila wadsworthia infection resulted in the reduction of body weight and fat mass, apparent hepatosplenomegaly and elevated serum inflammatory factors, including serum amyloid A and interleukin-6, while without significant change of the overall gut microbiota structure.ConclusionsThese results demonstrated that higher amount of B. wadsworthia caused systemic inflammatory response in SPF mice, which adds new evidence to the pathogenicity of this bacterium and implied its potential role to the chronic inflammation related metabolic diseases like diabetes.
Highlights
Bilophila wadsworthia is a major member of sulfidogenic bacteria in human gut, it was originally recovered from different clinical specimens of intra-abdominal infections and recently was reported potentially linked to different chronic metabolic disorders
This bacterium was detected over-represented in colonic microbiota of colorectal cancer patients, which implied its possible role in colorectal carcinogenesis [6]
Isolation and identification of B. wadsworthia The B. wadsworthia strain was isolated from fresh feces of a female volunteer who was newly diagnosed with latant autoimmune diabetes in adults (LADA)
Summary
Bilophila wadsworthia is a major member of sulfidogenic bacteria in human gut, it was originally recovered from different clinical specimens of intra-abdominal infections and recently was reported potentially linked to different chronic metabolic disorders. Devkota reported that a milk-derived saturated fat diet induced a bloom of B. wadsworthia in gut of SPF mice. Mono-inoculation of this pathobiont in germfree IL10−/− mice fed with milk fat diet can even induce TH1 immune response and colitis development [5]. This bacterium was detected over-represented in colonic microbiota of colorectal cancer patients, which implied its possible role in colorectal carcinogenesis [6]. Though mounting studies have highlighted the correlations of B. wadsworthia in different human diseases [7,8,9], especially chronic metabolic diseases, the mechanisms of its pathogenicity are not yet well characterized
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