Abstract
The periodontal ligament (PDL) is exposed to different kinds of mechanical stresses such as bite force or orthodontic tooth movement. A simple and efficient model to study molecular responses to mechanical stress is the application of compressive force onto primary human periodontal ligament fibroblasts via glass disks. Yet, this model suffers from the need for primary cells from human donors which have a limited proliferative capacity. Here we show that an immortalized cell line, PDL-hTERT, derived from primary human periodontal ligament fibroblasts exhibits characteristic responses to glass disk-mediated compressive force resembling those of primary cells. These responses include induction and secretion of pro-inflammatory markers, changes in expression of extracellular matrix-reorganizing genes and induction of genes related to angiogenesis, osteoblastogenesis and osteoclastogenesis. The fact that PDL-hTERT cells can easily be transfected broadens their usefulness, as molecular gain- and loss-of-function studies become feasible.
Highlights
The periodontal ligament (PDL) is a thin sheet-like structure surrounding teeth, serving to anchor teeth in alveolar bones
We found a significant increase of these markers during the time course of Primary For hPDLF
We investigated the expression of genes involved in formation of extracellular matrix (ECM) because they are known to be influenced in primary hPDLF by compressive force [16,25]
Summary
The periodontal ligament (PDL) is a thin sheet-like structure surrounding teeth, serving to anchor teeth in alveolar bones. It consists of different kinds of connective tissue fibers. This tissue has a high capacity of proliferation and of remodeling, enabling a constant width, despite different kinds of mechanical stress such as occlusal pressure and orthodontic force [1,2]. PDL fibroblasts are the main cells surrounding teeth, besides osteoblastic lineages constituting the bones.
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
