Abstract

Using Bowie's and other staining methods, histological examinations were made on seven kinds of endocrine and three kinds of exocrine glands of fish, and the fbllowing results were obtained. Bowie's method is recommended for demonstration of the cellular secretorty granules in BROCKMANN body, corpuscle of STANNIUS, renal juxtaglomerular cells, pancreatic acinar cells, and gastric zymogenie cells. Use of this method for differentiation gave excellent results in the secretory granules of A-and B-cells of Brockmann body. Furthermore, Bowie's method is suitable for staining the neuro-secretory inclusion in the preoptic neurosecretory cells, STH-and ACTH-cells of pituitary gland, and also colloid in the lumen of thyroid follicle. However, this method is not applicable for staining the chromaffin and interrenal cells of the adrenal gland, cells of uitimobranchial gland, and also the epidermal mucous cells of fish skin.

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