A histological investigation of the influence of Naringin on acetaminophen-induced nephrotoxicity in male albino rats

Abstract

A study was conducted to examine the protective effects of naringin (NRG) on renal tissues of rats, specifically against acetaminophen (ACN)-induced damage. Thirty-two male rats were included in the study, divided into four groups. The first group (group I) was administered 1 ml/kg of saline orally, serving as the control group. The second group received 2g/kg of ACN on the first day. The third group received a single oral dose of 2g/kg of ACN on the first day, followed by 40 mg/kg of oral NRG for eight consecutive days. Lastly, the fourth group was given a single oral dose of 2g/kg of ACN on the first day, followed by 80 mg/kg of oral NRG for eight days. The rats were then euthanized and their kidneys were removed after they had been anesthetized for blood collection. The ACN-induced renal damage was characterized by significant reductions in body weight, levels of superoxide dismutase (SOD), and catalase. Additionally, there were significant increases in kidney weight, serum creatinine, blood urea nitrogen, serum uric acid, malondialdehyde enzyme (MAD), and histopathological alterations. The study demonstrated that NRG treatment at doses of 40 and 80 mg/kg b.w. effectively reduced renal toxicity and oxidative damage induced by ACN. The effectiveness of these improvements was demonstrated by the noticeable increase in body weight, elevated levels of SOD and CAT, as well as the reduction in kidney weight, CR, BUN, SUA, and MAD levels. As a result of these findings, NRG is confirmed to have antioxidant properties and be capable of protecting rats from renal toxicity caused by ACN.