Abstract
Ovarian tissues from the ovoviviparous elasmobranch, Squalus acanthias were examined histochemically for the distribution of NAD and NADP diaphorase, glucose-6-phosphate dehydrogenase (G-6-PDH), 3 beta hydroxysteroid dehydrogenase (3βHSD), 3 alpha hydroxysteroid dehydrogenase (3αHSD), 17 beta hydroxysteroid dehydrogenase (17βHSD), and 20 beta hydroxysteroid dehydrogenase (20βHSD). The histochemical distribution of the enzymes was correlated with the histological picture obtained from contiguous sections. NAD, NADP diaphorase, and G-6-PDH were distributed throughout the ovary and positive reactions were obtained in all tissues tested. 3βHSD activity was demonstrated in the granulosa of follicles at various stages of development, increasing in intensity as the follicle matured. After ovulation, 3βHSD was found in the post-ovulatory follicle (“corpus luteum”). No 3βHSD activity was observed in atretic follicles (“preovulatory corpora lutea”). 3αHSD was only observed as a weak reaction in the granulosa of late preovulatory follicles, and no activity of 17βHSD and 20βHSD was observed in any ovarian component tissues.
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