Abstract
The remarkable features of aptamer as biological agent in detecting various compound with high binding affinity could be useful in monitoring of 17α-ethinylestradiol (EE2). EE2 is considered as an endocrine disrupting compound due its ecotoxicity and has widespread in the aquatic ecosystem due to improper disposal. Hence, there is a pressing requirement for sensitive and selective detection of EE2. In this study, we developed a new electrochemical biosensor based on modified DNA aptamers and gold nanoparticles/poly(n-butyl acrylate-co-N-acryloxysuccinimide) microspheres (Au/P(nBA-NAS)). The DNA aptamers was truncated from a 76-mer sequence to 39-mer sequences via computational method, has showed improved binding affinity and stability for EE2. The biosensor was fabricated by attaching the aptamers to the Au/P(NBA-NAS) microspheres, which is provided a large surface area and enhanced electron transfer for the immobilization and signal amplification of the aptamers. The detection principle of the [Fe(CN)6]−3/−4 redox probe in differential pulse voltammetry (DPV) measurement by the presence of aptamer-EE2 complexes on the electrode surface. The biosensor exhibited a linear relationship between the current decrease and the EE2 concentration in the range of 1 × 10−6 M to 1 × 10−12 M, with a detection limit of 1.7 × 10−13 M. The biosensor exhibited excellent selectivity, repeatability, and recovery for detecting EE2 in real samples, with results that closely matched those obtained using the high-performance liquid chromatography (HPLC) technique. This study provided a promising alternative for developing aptamer-based sensors for onsite environmental monitoring.
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