Abstract
A superparamagnetic iron oxide nanoparticle (SPION) was coupled to 2-aminoethyl-trimethyl ammonium (TMA) in a 2-step ligand exchange reaction to produce TMA–SPION. This particle, which has a strong positive charge, was investigated as the basis for a simple and efficient method for labelling human mesenchymal stem cells (hMSCs) for noninvasive monitoring by magnetic resonance imaging (MRI). TMA–SPION has a ζ potential of +40 mV and a hydrodynamic size of 101 nm. In addition to its long-term stability in an aqueous solution, TMA–SPION has a low cytotoxicity and favourable magnetic properties as a T2 contrast agent due to its high relaxivity. The T2 relaxivity of TMA–SPION is 4.4 times greater than the commercially available Feridex® I.V. magnetic resonance agent. Despite a short labelling time of 4 h, hMSCs are efficiently labelled with TMA–SPION without the need for a transfection agent. An in vivo MRI study of a brain infarction model confirmed the utility of TMA–SPION as an MRI tracking marker of administered hMSCs.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
