Abstract
Monoclonal antibodies (MAbs) were generated against human prostate specific antigen (PSA) to allow development of a sensitive free-PSA (f-PSA) assay. Of a total of 211, 12 could detect only f-PSA, the other 199 MAbs binding to both f-PSA and complex-PSA. In the present study, one MAb (no. 5C6) specific for f-PSA and another (no. 79) reacting with both forms were used to develop an enzyme-linked immunoassay (ELISA) for serum f-PSA. The detection limit was established as 0.008 μg/l ( n=20, mean of zero standard+3 S.D.) and the average recovery of f-PSA was 93–102%. The within-run and between-day coefficient of variation (CV) varied from 5.4–7.4% and 4.8–6.5%, respectively. The cross-reactivity of the assay to PSA-α 1-antichymotrypsin complex was determined to be <0.4%.
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