Abstract
The present research describes a simple method for the identification of the gender of arowana fish (Scleropages formosus). The DNA biosensor was able to detect specific DNA sequence at extremely low level down to atto M regimes. An electrochemical DNA biosensor based on acrylic microsphere-gold nanoparticle (AcMP-AuNP) hybrid composite was fabricated. Hydrophobic poly(n-butylacrylate-N-acryloxysuccinimide) microspheres were synthesised with a facile and well-established one-step photopolymerization procedure and physically adsorbed on the AuNPs at the surface of a carbon screen printed electrode (SPE). The DNA biosensor was constructed simply by grafting an aminated DNA probe on the succinimide functionalised AcMPs via a strong covalent attachment. DNA hybridisation response was determined by differential pulse voltammetry (DPV) technique using anthraquinone monosulphonic acid redox probe as an electroactive oligonucleotide label (Table 1). A low detection limit at 1.0 × 10−18 M with a wide linear calibration range of 1.0 × 10−18 to 1.0 × 10−8 M (R2 = 0.99) can be achieved by the proposed DNA biosensor under optimal conditions. Electrochemical detection of arowana DNA can be completed within 1 hour. Due to its small size and light weight, the developed DNA biosensor holds high promise for the development of functional kit for fish culture usage.
Highlights
Asiatic arowana (Scleropages formoss), a freshwater fish, [1] is widely distributed over the countryside of Southeast Asia region such as Malaysia, Singapore, Thailand, Indonesia, Cambodia, Vietnam, Laos, Myanmar and the Philippines
Stock solution of DNA probe was diluted with 0.05 M of Kphosphate buffer while complementary DNA and non-complementary solutions were prepared with 0.05 M of Na-phosphate buffer at pH 7.0 containing 1.0 mM of AQMS
The effect of the different scan rates of the carbon screen printed electrode (SPE) containing acrylic microspheres (AcMPs)-AuNPs in the presence of K3Fe(CN)6 showed that the oxidation and reduction peak currents increased with the increasing of the scan rate from 0.05 to 0.30 V/s (Fig. 4)
Summary
Asiatic arowana (Scleropages formoss), a freshwater fish, [1] is widely distributed over the countryside of Southeast Asia region such as Malaysia, Singapore, Thailand, Indonesia, Cambodia, Vietnam, Laos, Myanmar and the Philippines. The arowana fish is found in Australia and New Guinea [1–4]. It is popularly known as dragonfish, Asia bonytongue, kelisa, or baju-rantai [5, 6]. It is still surviving as a primitive fish species from the Jurassic era [7, 8]. The Chinese and Asian people considered it as a symbol of. Due to its high popularity and great demand in ornamental purposes, Asian arowana has been fiercely hunted for profits [6], and results in a rapid decline of its population. Considering its high demand in ornamental industry, the over-exploitation of natural populations, and the rarity of Rahman et al Nanoscale Research Letters (2017) 12:484
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