A highly selective optical sensor Eu-BINAM for assessment of high sensitivity cardiac troponin tumor marker in serum of cancer patients

Abstract

A novel, easy, touchy and selective spectrofluorimetric technique has been successfully applied for sensitive determination of High Sensitivity Cardiac Troponin (TNHS I) in the serum samples of patients suffering malignant tumors through the usage of optical sensor Eu3+-BINAM complex. The technique is primarily based on quenching of the Eu3+-BINAM complex's luminescence intensity upon introducing various concentrations of High Sensitivity Cardiac Troponin (TNHS I). The synthesis and characterization of the optical sensor was performed via absorption and emission. The sensor was also adapted to offer excitation at 394 nm in acetonitrile at pH 7.5. Concentration of High Sensitivity Cardiac Troponin (TNHS I) in serum samples was found to be proportional to the luminescence intensity quenching of the Eu3+-BINAM complex, most prominently at λem = 618 nm. The limit of the dynamic range is 4.26 × 10−4 to 2 ng/mL. The limit of detection and quantitation were calculated to be 1.35 and 4.10 ng/mL, respectively. The suggested analytical approach proved its applicability, simplicity and comparatively interference- free. The technique was effectively recruited to quantify High Sensitivity Cardiac Troponin (TNHS I) in human serum samples. The proposed technique could be further extended to evaluate some biomarkers associated with malignancy related diseases in human.