Abstract
Stem cell research has gathered immense attention in the past decade due to the remarkable ability of stem cells for self-renewal and tissue-specific differentiation. Despite having numerous advancements in stem cell isolation and manipulation techniques, there is a need for highly reliable probes for the specific detection of live stem cells. Herein we developed a new fluorescence probe (CDy9) with high selectivity for mouse embryonic stem cells. CDy9 allows the detection and isolation of intact stem cells with marginal impact on their function and capabilities.
Highlights
Despite the potential of stem cells for the treatment of complex diseases, there are still no effective ways to detect stem cells in vivo or ex vivo. This is mainly due to their heterogenetic nature and unpredictable pattern of proliferation and differentiation in ex vivo cultures.[2]
We report the identification of a fluorescent small molecule with high specificity for mouse embryonic stem cells (mESC) and marginal staining in a whole range of cells derived from different lineages of the three germ layers
From our high-throughput cell imaging screening, we identified CDy9 as a fluorescent small molecule with high selectivity for mESC over mouse embryonic fibroblasts (MEF) (Fig. 1)
Summary
A highly selective fluorescent probe for direct detection and isolation of mouse embryonic stem cells Yogeswari Chandran a, Nam-Young Kang a, Sung-Jin Park a, Samira Husen Alamudi b, Jun-Young Kim a, Srikanta Sahu a, Dongdong Su a, Jungyeol Lee b, Marc Vendrell c,⇑, Young-Tae Chang a,b,⇑
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