A highly selective and recyclable NO-responsive nanochannel based on a spiroring opening\u2212closing reaction strategy

Yao Sun,Guangfu Yang,Haibing Li,Xiaoya Chen,Sen Chen,Siyun Zhang,Yuling Xu,Qingying Ouyang

doi:10.1038/s41467-019-09163-4

Yao Sun, Guangfu Yang + Show 6 more

Open Access

https://doi.org/10.1038/s41467-019-09163-4

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Abstract

Endogenous nitric oxide (NO) is an important messenger molecule, which can directly activate K+ transmission and cause relaxation of vascular smooth muscle. Here, inspired by the K+ channel of smooth muscle cells, we report, a novel NO-regulated artificial nanochannel based on a spiro ring opening−closing reaction strategy. This nanofluidic diode system shows an outstanding NO selective response owing to the specific reaction between o-phenylenediamine (OPD) and NO on the channel surface with high ion rectification ratio (~6.7) and ion gating ratio (~4). Moreover, this NO gating system exhibits excellent reversibility and stability as well as high selectivity response. This system not only helps us understand the process of NO directly regulating biological ion channels, but also has potential application value in the field of biosensors.

Highlights

Endogenous nitric oxide (NO) is an important messenger molecule, which can directly activate K+ transmission and cause relaxation of vascular smooth muscle
Inspired by the NO-activated K+ channel in nature, we report the utilization of a reversible covalent reaction strategy to fabricate a nanochannel-responsive system that exhibits high NO specificity and stability (Fig. 1)
After successful construction of the nanofluidic diode, we investigated the feasibility of the nanochannel in response to NO

Summary

Introduction

Endogenous nitric oxide (NO) is an important messenger molecule, which can directly activate K+ transmission and cause relaxation of vascular smooth muscle. Inspired by the K+ channel of smooth muscle cells, we report, a novel NO-regulated artificial nanochannel based on a spiro ring opening−closing reaction strategy. This nanofluidic diode system shows an outstanding NO selective response owing to the specific reaction between o-phenylenediamine (OPD) and NO on the channel surface with high ion rectification ratio (~6.7) and ion gating ratio (~4). This NO gating system exhibits excellent reversibility and stability as well as high selectivity response This system helps us understand the process of NO directly regulating biological ion channels, and has potential application value in the field of biosensors. Functional single conical artificial nanochannels have been actively explored to mimic their bio-counterparts because of their excellent robust mechanical and chemical properties[13,14,15,16,17,18,19,20,21,22]

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