A Highly Porous Sodium Dodecyl Sulfate-Polyacrylamide Gel System

Abstract

The major contributions to the development of protein separation by gel electrophoresis were the introduction of polyacrylamide gels (Raymond and Weintraub, 1959) and of discontinuous buffer systems (Davis, 1964; Ornstein, 1964), the use of the powerful detergent sodium dodecyl sulfate (SDS) to solubilize protein complexes prior to electrophoresis (Summers et al., 1965), and the addition of SDS to discontinuous buffer systems (Laemmli, 1970; Neville, 1971). In 1977, Porzio and Pearson (1977) described a highly porous SDS-polyacrylamide gel which gave an increased resolution over a wide range of polypeptide molecular weights (MWs). This latter contribution was originally introduced as a continuous disk electrophoresis method.