Abstract
Wine and grape juice are known to have hydroxylated stilbenes, a group of phytoalexins, health-promoting compounds. The determination of stilbene species in wine and grape juices remains a challenging task. Here, we propose an efficient strategy for the simultaneous determination of eleven hydroxylated stilbenes in grape wines and grape juice by a SALLE in conjunction with an HPLC-ESI-MS/MS. SALLE and HPLC-MS/MS conditions were optimized. The proposed method was validated and found to be a simple, sensitive, and reliable measure for the determination of the stilbene species in wine and grape juice. The SALLE was fast and efficient, taking only 10min. The HPLC-MS/MS was able to quickly separate and quantitate the eleven stilbenes (9min running). This method was successfully applied to determine the contents of stilbene species in commercial wine and grape juice in Korea. This method is advantageous in reduced sample preparation time, low labor, and cost efficiency.
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