Abstract

Radiation induced graft polymerization (RIGP) is an excellent tool for functionalization of polymers, allowing the preparation of high density polymer brushes suitable for enzyme immobilization. Herein, we report on a hollow fiber membrane bioreactor prepared using RIGP to host laccase which is an efficient enzyme for removal of phenolic compounds. Polymer brushes were functionalized with aldehyde moieties to allow covalent bonds in between the polymer and the enzyme for immobilization. Moisture retention was also greatly improved after functionalization. As a result, after optimization of immobilization and reaction conditions, the bioreactor showed high stability in organic media and efficient biodegradation of an organic phenolic pollutant (bisphenol A).

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