Abstract
Bioproduction of 5-aminovalerate (5AVA) from renewable feedstock can support a sustainable biorefinery process to produce bioplastics, such as nylon 5 and nylon 56. In order to achieve the biobased production of 5AVA, a 2-keto-6-aminocaproate-mediated synthetic pathway was established. Combination of L-Lysine α-oxidase from Scomber japonicus, α-ketoacid decarboxylase from Lactococcus lactis and aldehyde dehydrogenase from Escherichia coli could achieve the biosynthesis of 5AVA from biobased L-Lysine in E. coli. The H2O2 produced by L-Lysine α-oxidase was decomposed by the expression of catalase KatE. Finally, 52.24 g/L of 5AVA were obtained through fed-batch biotransformation. Moreover, homology modeling, molecular docking and molecular dynamic simulation analyses were used to identify mutation sites and propose a possible trait-improvement strategy: the expanded catalytic channel of mutant and more hydrogen bonds formed might be beneficial for the substrates stretch. In summary, we have developed a promising artificial pathway for efficient 5AVA synthesis.
Highlights
Increasing concerns over global water pollution, climate change, public health, and petroleum shortages have attracted considerable attention to sustainable development as promising green alternatives to traditional petrochemical-derived chemicals renewable feedstock (Tsuge et al, 2016)
As a kind of green alternative to petrochemical products, microbial bioplastics are composed of monomers containing appropriate functional groups, which have become the focus of metabolic engineering research
It is worth mentioning that two straight-chain amino acids—5-aminovalerate (5AVA) and 4-aminobutyrate— are promising platform compounds for the synthesis of polyimides, serving as raw materials for disposable goods, clothes and automobile parts like nylon 5 (Adkins et al, 2013) and nylon 4 (Park et al, 2013a) because of its high temperature and organic solvent resistance
Summary
Increasing concerns over global water pollution, climate change, public health, and petroleum shortages have attracted considerable attention to sustainable development as promising green alternatives to traditional petrochemical-derived chemicals renewable feedstock (Tsuge et al, 2016). As a kind of green alternative to petrochemical products, microbial bioplastics are composed of monomers containing appropriate functional groups, which have become the focus of metabolic engineering research. These compounds include amino acids such as methionine (Kromer et al, 2006) and leucine. It is worth mentioning that two straight-chain amino acids—5-aminovalerate (5AVA) and 4-aminobutyrate— are promising platform compounds for the synthesis of polyimides, serving as raw materials for disposable goods, clothes and automobile parts like nylon 5 (Adkins et al, 2013) and nylon 4 (Park et al, 2013a) because of its high temperature and organic solvent resistance
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