Abstract

In this research, novel biorefinery processes for obtaining value-added chemicals such as biosugar and hesperidin from mandarin peel waste (MPW) are described. Herein, three different treatment methods were comparatively evaluated to obtain high yields of biosugar and hesperidin from MPW. Each method was determined by changes in the order of three processing steps, i.e., oil removal, hesperidin extraction, and enzymatic hydrolysis. The order of the three steps was found to have a significant influence on the production yields. Biosugar and hesperidin production yields were highest with method II, where the processing steps were performed in the following order: oil removal, enzymatic hydrolysis, and hesperidin extraction. The maximum yields obtained with method II were 34.46 g of biosugar and 6.48 g of hesperidin per initial 100 g of dry MPW. Therefore, the methods shown herein are useful for the production of hesperidin and biosugar from MPW. Furthermore, the utilization of MPWs as sources of valuable materials may be of considerable economic benefits and has become increasingly attractive.

Highlights

  • IntroductionEnvironmental pollution is among the most serious problems faced by humanity today [1]

  • Environmental pollution is among the most serious problems faced by humanity today [1].The United Nations Food and Agriculture Organization (FAO) has estimated that approximately1.3 billion tons of food is wasted worldwide every year, with fruits and vegetables having the highest wastage rates among all the food types [2]

  • If the enzyme loading can be reduced, the cost of enzyme will reduce naturally. These results reveal that pectinase and cellulase acted synergistically during the hydrolysis of the mandarin peel waste (MPW)

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Summary

Introduction

Environmental pollution is among the most serious problems faced by humanity today [1]. Is used extensively in the pharmaceutical and food industries, and its demand is Thehesperidin global hesperidin market was valued at USD 81 million in 2019 and is expected to reach USD increasing worldwide [22,23]. This high concentration and the large amount of peel waste available render concentrations of flavonoids such as hesperidin in the mandarin fruit [25] Andathe amount of peeloccurring waste available render MPW a rich source of naturally occurring The development of an effective and selective method for bioactive compound extraction would flavonoids. Biosugars can be used as raw materials to obtain carbon sources for fermentation processes, which are used to produce high-value products [35].

Design of Experimental
Methods
Optimization of Enzyme Loading
Biosugar Production via Enzymatic Hydrolysis
Overall
The production efficiencies ofIthe three methods
Materials
Carbohydrate Analysis
Enzymatic Hydrolysis
Removal of Oil Fraction from MPW
Methanol Extraction of Hesperidin
Purification of Hesperidin
Characterization of Hesperidin
Conclusions
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