A High-Throughput Strategy for Glycine Oxidase Biosensor Development Reveals Glycine Release from Cultured Cells

Abstract

Glycine is an important biomarker in clinical analysis due to its involvement in multiple physiological processes. As such, the need for low-cost analytical tools for glycine detection is growing. As a neurotransmitter, glycine is involved in inhibitory and excitatory neurochemical transmission in the central nervous system. In this work, we present a 10 μM Pt-based electrochemical enzymatic biosensor for localized real time measurements of glycine. This biosensor relies on amperometric readout and does not require additional redox mediators. The biosensor was characterized and applied for real-time glycine detection from cells, mainly HEK 293 cells and primary rat astrocytes. Taken together, this study demonstrates a glycine oxidase based biosensor useful for extracting pertinent information about glycine levels in a wide range of biological contexts.