Abstract
Screening for microorganisms with antagonistic activity against crop pathogens is essential for microbial antagonist preparation, crop disease control, and sustainable agricultural development. This study established a rapid, efficient, one-step high-throughput method for screening endophytic bacteria displaying antagonistic activity against Magnaporthe oryzae (M. oryzae) ACCC36020 in rice seeds. The experimental results indicated that these bacteria could be directly screened from six sample groups using this method. This technique can achieve simultaneous screening and purification, identify endophytic bacteria displaying antifungal activity in rice seeds, and save significant time.
Highlights
Rice blast caused by M. oryzae is a worldwide rice disease, which causes a huge loss of rice yield every year (Feng et al 2019)
A rapid, efficient, and one-step highthroughput method for screening endophytic bacteria with antagonistic activity against Magnaporthe oryzae in rice seeds was successfully established, and the experimental results showed that the endophytic bacteria with antagonistic activity of Magnaporthe oryzae Agricultural Culture Collection of China (ACCC) 36020 could be directly screened from six groups of experimental samples by this method
The experimental results showed that the endophytic bacteria with the activity for resistance to M. oryzae ACCC 36020 were successfully screened from the six varieties of rice seed samples after using the experimental method we designed (Fig. 1 and Fig. 2)
Summary
Rice blast caused by M. oryzae is a worldwide rice disease, which causes a huge loss of rice yield every year (Feng et al 2019). In order to realize efficient and high-throughput screening of endophytic bacteria with antagonistic fungal activity in rice seeds, we selected 6 rice varieties with different resistance to rice blast as materials and established a highthroughput screening method for endophytic bacteria with the antagonistic activity of M. oryzae in rice seeds through experiments.
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