Abstract
Lipidomics is of increasing interest in studies of biological systems. However, high-throughput data collection and processing remains non-trivial, making assessment of phenotypes difficult. We describe a platform for surveying the lipid fraction for a range of tissues. These techniques are demonstrated on a set of seven different tissues (serum, brain, heart, kidney, adipose, liver, and vastus lateralis muscle) from post-weaning mouse dams that were either obese (> 12 g fat mass) or lean (<5 g fat mass). This showed that the lipid metabolism in some tissues is affected more by obesity than others. Analysis of human serum (healthy non-pregnant women and pregnant women at 28 weeks’ gestation) showed that the abundance of several phospholipids differed between groups. Human placenta from mothers with high and low BMI showed that lean placentae contain less polyunsaturated lipid. This platform offers a way to map lipid metabolism with immediate application in metabolic research and elsewhere.Graphical abstract
Highlights
Detailed molecular surveys of the lipid fraction of biological samples are of increasing interest in research of metabolism, development, and disease
The design of the pipeline incorporates several novel features for characterizing the lipid fraction of biological samples at the molecular level. This pipeline has been used on a set of mouse tissues and two human sample types in order to indicate its capabilities and where special attention is required in applying the platform or individual steps to high-throughput studies
In order to profile the fatty acids (FAs) in the phospholipid fraction, we have extended this to include a third mode that fragments species that ionise in negative mode
Summary
Detailed molecular surveys of the lipid fraction of biological samples (lipidomics) are of increasing interest in research of metabolism, development, and disease. There is mounting evidence that lipid metabolism has a crucial role in growth and metabolic disease in humans [12,13,14,15,16] This has encouraged the development of methods required for lipid profiling. The interest has led to questions that cannot be answered by determining the lipid composition of the circulating plasma or serum alone Answering such questions requires an understanding of the lipid metabolism within and between organs, such as the liver and brain, across the life-course. This requires lipid profiling of more than one tissue, often at the same time, from the same individual animal and even longitudinally
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