Abstract
A high throughput, relatively rapid, reliable and inexpensive protocol for the genomic DNA isolation from chickpea is described. The procedure involved a modified cetyltrimethyl-ammonium bromide (CTAB) of and β-mercaptoethanol buffer protocol with the use of indigenous genogrinder for DNA extraction from 100 mg of mature and young leaves of chickpea. The present protocol is also well suited for extracting high-molecular weight DNA from leguminous plant materials rich in polyphenols, tannins and polysaccharides. The DNA obtained by the present protocol is of very good quality, with no coloured pigment and contaminants, without using expensive liquid nitrogen and toxic phenols.
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