Abstract
The large amount of existing nanomaterials demands rapid and reliable methods for testing their potential toxicological effect on human health, preferably by means of relevant in vitro techniques in order to reduce testing on animals. Combining high throughput workflows with automated high content imaging techniques allows deriving much more information from cell-based assays than the typical readouts (i.e. one measurement per well) with optical plate-readers. We present here a dataset including data based on a maximum of 14 different read outs (including viable cell count, cell membrane permeability, apoptotic cell death, mitochondrial membrane potential and steatosis) of the human hepatoma HepaRG cell line treated with a large set of nanomaterials, coatings and supernatants at different concentrations. The database, given its size, can be utilized in the development of in silico hazard assessment and prediction tools or can be combined with toxicity results from other in vitro test systems.
Highlights
Background & SummaryThe increasing use of manufactured nanomaterials (MNMs) in food, industrial processes and consumer products poses a significant challenge to industry and regulatory bodies with regard to demonstrating their safety[1,2,3,4]
We present here a dataset including data based on a maximum of 14 different read outs of the human hepatoma HepaRG cell line treated with a large set of nanomaterials, coatings and supernatants at different concentrations
An additional aim was to use the dataset to explore the development of MNM-specific Quantitative Property Activity Relationships (QPARs) by combining the toxicity data with the physiochemical properties of the MNMs
Summary
Background & SummaryThe increasing use of manufactured nanomaterials (MNMs) in food, industrial processes and consumer products poses a significant challenge to industry and regulatory bodies with regard to demonstrating their safety[1,2,3,4]. In addition to the tested items, each plate contained at least four negative control wells where cells were treated only with medium, and a number of wells with chemical controls.
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